Abstract
The inappropriate and excessive use of pesticides poses a great challenge to human health. It is necessary to analyze pesticide residues efficiently, economically and quickly to minimize the risk of public safety issues. Herein, a dual-mode biosensor was proposed for ultrafast and ultratrace detection of acetamiprid (ACE). The polypyrrole nanowires (PPyNWs) was prepared using the guidance of CS by electrochemical method, which showed large electroactive surface area, excellent conductivity and high stability. After immobilization of ACE aptamer onto CS/PPyNWs, the biosensor can be effortlessly prepared. The ACE concentration can be measured by both the signal decrease of the chronocoulometry (CC) method and the chronoamperometry (CA) method. The dual-mode biosensor exhibited ultrafast detection speeds of 0.5 s and 2 s across 9 and 6 orders of magnitude linear ranges through CC and CA, respectively. More importantly, the biosensor was successfully applied to detect ACE residues in soil samples, proving the feasibility of the dual-mode biosensor for ACE detection in complex samples.
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