Abstract
We describe a dual-modality laser scanning endomicroscope that provides simultaneous fluorescence contrast based on confocal laser endomicroscopy (CLE) and phase-gradient contrast based on scanning oblique back-scattering microscopy (sOBM). The probe consists of a 2.6mm-diameter micro-objective attached to a 30,000-core flexible fiber bundle. The dual contrasts are inherently co-registered, providing complementary information on labeled and un-labeled sample structure. Proof of principle demonstrations are presented with ex-vivo mouse colon tissue.
Highlights
Fluorescence endomicroscopy has garnered attention in recent years for its ability to provide histology-like high resolution images in situ and in real time
To address the above limitations we introduced oblique back-illumination microscopy (OBM), which produces trans-illumination-based phase-gradient contrast in an en-face geometry, meaning it can be used with arbitrarily thick samples [31]
We showed that OBM can be configured in an endomicroscopy configuration with a flexible fiber bundle [32], where it produces speckle-free images of slowly varying tissue structures that would be impossible to observe with reflection-based contrast
Summary
Fluorescence endomicroscopy has garnered attention in recent years for its ability to provide histology-like high resolution images in situ and in real time. “Confocal microscopy through a fiber-optic imaging bundle," Opt. Lett. “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle," J.
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