Abstract
BackgroundDual loading drug delivery system with tumor targeting efficacy and sequential release function provides a promising platform for anticancer drug delivery. Herein, we established a novel AuCOOH@FACS nanogel system for co-delivery miR-218 mimics (as bio-drug) and Temozolomide(as chemo-drug).MethodsDLS and TEM were employed to determine the characteristics of particles and nanogels. The cell viability was calculated for study synergistic effect of both drugs coadministration and in nanogel forms. The amounts of Au uptake were measured by ICP-MS in cell and tumors to quantify the targeting drug delivery efficacy. Tumor weight and mice weight were investigated to study the targeting antitumor efficacy of nanogel system.ResultsThe results revealed that using AuCOOH@FACS nanogel as delivery vehicles, drugs could be targeting delivery to tumor site, the intracellular uptake is enhanced to a greater extent, and significant antitumor efficacy is fold increase compared with free drug administration group, without noticeable system cytotoxicity.ConclusionsThis system offers an efficient approach to cancer therapy and holds significant potential to improve the treatment of cancer in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-015-0216-8) contains supplementary material, which is available to authorized users.
Highlights
Nanoscaled drug carriers have been used widely for drug delivery such as liposomes [1,2,3], microspheres [4, 5], polymeric shells, etc. [6]
In our previous study, we found that overexpression of miR-218 in glioma cells markedly suppresses the motility, invasion, and proliferation of glioma cells [18, 19], so miR-218 mimics was attached on AuCOOH_miR-218 mimics MUA capped gold nanoparticles (AuCOOH) (AuCOOH_miR218 mimics) surface as bio drug
UV absorption spectra were taken from the supernatant to measure the amount of Temozolomide released at certain time intervals
Summary
Preparation of 11-Mercaptoundecanoic acid (MUA) capped anionic gold nanoparticles (AuCOOH) and AuCOOH_miR-218 mimics MUA capped gold nanoparticles (AuCOOH) were prepared from pentane thiol-capped gold NPs (~2 nm core) via a place exchange reaction. The black precipitation of AuCOOH_miR-218 mimics was further dialysis for 2 days to remove free ligands, NPs were lyophilized and redissolved in MilliQ water. Cells were seeded at initial densities of 5 × 104 cells/mL (2 ml culture media per dish) in dishes and incubated for 24 h, and changed the medium with AuCOOH_miR-218 mimics@FACS nanogel (2 ml). The cells were washed with PBS and incubated with AuCOOH miR-218 mimics, AuCOOH_miR-218 mimics@CS and AuCOOH_miR-218 mimics@FACS (150nM Gold nanoparticles in chitosan) for 6 h cells were washed three times with PBS and lysed. Stability of AuCOOH_miR-218 mimics NPs, AuCOOH_miR-218 mimics@CS, and AuCOOH_miR-218 mimics@FACS nanogels were investigated by incubating samples in H2O, PBS and medium with serum for 24 h (Additional file 1: Figure S1). The size distribution of nanogels was revealing no
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