Dual inhibition of cyclooxygenase-1 and 5-lipoxygenase by aerial part of Bupleurum fruticescens methanol extract

Abstract

The effect of the methanol extract from aerial parts of Bupleurum fruticescens on the release of eicosanoids and hydrolytic enzymes was determined on in vitro cell systems . The extract had a significant effect on 5-lipoxygenase (5-LOX) activity, inhibiting both LTB 4 and 5( S)-HETE production with IC 50 values of 112 μg/ml and 95 μg/ml, respectively. At concentrations of 200 μg/ml, the extract also inhibited cyclooxygenase-1 (90%) and elastase activities (54%). The 12-LOX activity in intact platelets was not affected; a fact, which suggests that phospholipase A 2 (PLA 2) activity, is not modified by the extract.