Abstract
ATP produced whole-cell potassium currents with a latency of 200 msec in cultured smooth muscle cells of bovine brain arteries. The currents were evoked via an ATP receptor linked to a pertussis toxin-insensitive G-protein, which is not involved in phospholipase C (PLC)-mediated signal transduction, and were not regulated by protein kinase A or C. In the cell-attached patches, ATP elicited single channel currents with two major classes of slope conductances and these currents were again induced within 100 msec after bath-application of ATP outside the patch pipette. In addition, ATP enhanced intracellular free Ca 2+ concentration ([Ca 2+]i) with no initiation time in the presence and absence of extracellular Ca 2+, and this enhancement was not blocked by a PLC inhibitor. These results, thus, suggest that the activation of the potassium channel and the enhancement of [Ca 2+]i induced by ATP may be caused by a direct action of the G-protein βγ subunits.
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