Dual Determination of VO <sup>2+</sup>/Fe <sup>3+</sup> in Biological Samples Based on Citrate Doped Polypyrrol as a Selective Fluorescent Probe

Abstract

Vanadyl sulfate has been widely utilized in medical field due to its physiological performance like muscles improvement, insulin mimetic activity and antitumor properties. Herein, we have developed a facile and highly selective fluorescence probe for dual detection of VO2+ and Fe3+ in aqueous and biological media using citric acid doped polypyrrole as a highly bright fluorescence sensor. The PPy-Cit was shown different time quenching response to VO2+ and Fe3+ ions. The emission of PPy-Cit was quenched very quickly in response to VO2+, while Fe3+ quenched the probe after five minutes. This probe was then applied for VO2+ and Fe3+ determination in human serum and vanadyl sulfate tablet samples. The response of the probe was linear over the concentration from 1 to 100 µM and 5-80 µM with a detection limit of 340 and 590 nM for VO2+ and Fe3+ respectively in optimum experimental conditions. The results obtained by the PPy-Cit probe were in good agreement with ICP-EOS data. According to what we know, it is the first research regarding the use of fluorescence probe applies for VO2+ detection in a human serum providing a potential and promising strategy for rapid screening of VO2+ in presence of Fe3+ in biological fluids.