Abstract
Structured illumination microscopy (SIM) is one of the most widely used imaging tools in live-cell imaging because of the fast imaging speed and low-intensity requirement. In order to observe the interactions between cell structures, it is necessary to achieve multi-color simultaneous rapid imaging. However, existing systems collect multi-color results in a time-sequential manner. In live-cell imaging, this method brings distortions of the result. In this paper, we provide a dual-color simultaneous SIM system based on galvanometers, which can control the structured pattern of the two-channel flexibly. The resolution capability and simultaneous imaging capability of this system are verified, and the imaging results of living cells are obtained.
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