Abstract
A double cannulation technique which provides access to vascular and cerebroventricular compartments of the unanesthetized rat is described. The technique requires a short surgical preparation time (10–20 min). Plasma levels of luteinizing hormone, follicle stimulating hormone, and prolactin were found to be normal in male rats twenty-four hours after implantation of the cannulas. Plasma hormone levels were also not affected by intraventricular infusion of saline or by repeated blood withdrawal. The application of this technique to research in reproductive physiology and neuroendocrinology is proposed.
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