Dual boronate affinity nanoparticles-based plasmonic immunosandwich assay for specific and sensitive detection of ginsenosides.

Abstract

Ginsenoside is a large family of triterpenoid saponins from Panax ginseng with various important biological functions. It is crucial to develop effective analytical approach for qualitative and quantitative analysis of ginsenosides. Herein, a dual boronate affinity nanoparticles-based plasmonic immunosandwich assay has been developed for analysis of ginsenosides. An imprinted Au NPs-coated glass slide was prepared via controllable oriented surface imprinting and used as specific extraction substrate for target molecules. In the meantime, Ag-cored Raman nanotags were used for specific labeling of target molecules. The MIP-based recognitions ensured the specificity of the assay, while enhanced Raman signal derived from the imprinted substrate-target-nanotags sandwich-like complexes provided high sensitivity. The proposed immunosandwich assay exhibited wide linear range (10ng/mL to 10μg/mL), high sensitive (LOD: 1.7ng/mL, LOQ: 5ng/mL) and good reproducibility (RSD: 8.6%). For real-world applications, successful quantitative analysis of ginsenoside Re in ginseng was performed. Therefore, this dual boronate affinity nanoparticles-based plasmonic immunosandwich assay holds great promise in many important applications such as pharmaceutical analysis.