Abstract
Dual-band Fourier domain optical coherence tomography (FD-OCT) provides depth-resolved spectroscopic imaging that enhances tissue contrast and reduces image speckle. However, previous dual-band FD-OCT systems could not correctly give the tissue spectroscopic contrast due to depth-related discrepancy in the imaging method and attenuation in biological tissue samples. We designed a new dual-band full-range FD-OCT imaging system and developed an algorithm to compensate depth-related fall-off and light attenuation. In our imaging system, the images from two wavelength bands were intrinsically overlapped and their intensities were balanced. The processing time of dual-band OCT image reconstruction and depth-related compensations were minimized by using multiple threads that execute in parallel. Using the newly developed system, we studied tissue phantoms and human cancer xenografts and muscle tissues dissected from severely compromised immune deficient mice. Improved spectroscopic contrast and sensitivity were achieved, benefiting from the depth-related compensations.
Highlights
Optical coherence tomography (OCT) is a non-invasive imaging technique that provides high speed cross sectional images in biological tissues [1]
The intensity images from two wavelength bands are averaged as a frequency compound image for speckle reduction [9], and the two intensity images are subtracted as a differential image that provides spectroscopic information
To validate the new algorithm, we studied tissue phantoms and human PC-3 prostate cancer xenograft tumor and muscle tissues dissected from severely compromised immune deficient (SCID) mice
Summary
Optical coherence tomography (OCT) is a non-invasive imaging technique that provides high speed cross sectional images in biological tissues [1]. The frequency compound image and the differential image can be encoded in a single color image, straightforwardly showing both the de-speckled intensity and spectroscopic information. The depth-related fall-off [11] in FDOCT exacerbates a spectroscopic deviation. These problems generally exist in the dualband FD-OCT and are independent of color schematics. Previous dual-band FDOCT imaging systems could not correctly give the tissue spectroscopic information. To solve these problems, both hardware and software should be modified in the dual-band FD-OCT imaging system. We designed a new dual-band full-range FD-OCT system, and incorporated a depth-related compensation algorithm to correct spectroscopic data. To validate the new algorithm, we studied tissue phantoms and human PC-3 prostate cancer xenograft tumor and muscle tissues dissected from severely compromised immune deficient (SCID) mice
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