Abstract
Because of the side-effects of commonly used anti-platelet and anticoagulant drugs, investigations into plant substances with similar activities are very common. Based on our own studies in recent years, we estimate that it is possible to use natural compounds to both inhibit coagulation pathway enzymes and to reduce blood platelets’ activation. As such, in our current study we wanted to verify the anti-platelet and anticoagulant properties of grape seed extract (GSE) using in vitro models. During our analysis, the following parameters were analyzed: Coagulation times, thromboelastometry assays (coagulation time, clot formation time and maximum clot firmness), aggregation of platelets and phosphorylation of vasodilator-stimulated phosphoprotein (VASP). Adenosine diphosphate (ADP)-induced aggregation was lower in GSE 7.5 µg/mL as well as in GSE 15.0 µg/mL. A similar dependence was observed in VASP assays for GSE 7.5 µg/mL and GSE 15 µg/mL. The effect on plasma coagulation tests was distinct only with GSE 15 µg/mL. All of the thromboelastometry variables were statistically significant with 15.0 µg/mL GSE concentration. Our results show, for the first time, the multi-potential effect of grape seed extract on coagulation systems, and clearly suggest that grape seed extract could be considered a promising nutraceutical in the prevention of cardiovascular thrombotic events caused by different mechanisms.
Highlights
Antiplatelet and anticoagulant drugs have been the major players in their clinical setting [1,2]. their efficacy is well established, their deleterious, life-threatening side-effects have been well documented [3,4] which has prompted investigations into natural alternatives.An exploratory trial to identify new anticoagulants and anti-platelets sourced from medicinal plants is worthwhile
For the first time, the multi-potential effect of grape seed extract on coagulation systems, and clearly suggest that grape seed extract could be considered a promising nutraceutical in the prevention of cardiovascular thrombotic events caused by different mechanisms
We observed that incubation that incubation of whole blood with grape seed extract (GSE) decreased Adenosine diphosphate (ADP)-induced aggregation in a 7.5 μg/mL
Summary
Antiplatelet and anticoagulant drugs have been the major players in their clinical setting [1,2]. their efficacy is well established, their deleterious, life-threatening side-effects have been well documented [3,4] which has prompted investigations into natural alternatives.An exploratory trial to identify new anticoagulants and anti-platelets sourced from medicinal plants is worthwhile. Antiplatelet and anticoagulant drugs have been the major players in their clinical setting [1,2]. Their efficacy is well established, their deleterious, life-threatening side-effects have been well documented [3,4] which has prompted investigations into natural alternatives. Plants are a good source for isolating natural compounds without unfavorable side effects, capable of inhibiting coagulation pathway enzymes and reducing blood platelet activation [5,6,7]. Epidemiological studies have provided evidence that diets rich in polyphenols, such as flavonoids, protect against vascular dysfunction, promote vascular health and reduce the risk of cardiovascular and cerebrovascular diseases [8,9,10]. Polyphenol-rich diets have been shown to have a positive effect on the vascular system, mainly through their effects on platelets and endothelial functions [11,12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
