Abstract
Purified DT diaphorase from rat liver can support the aerobic oxidation of external DPNH and TPNH by isolated mitochondria when low concentrations of vitamin K 3 (2-methyl-1,4-naphthoquinone) are added. The oxidation is sensitive to dicoumarol which inhibits DT diaphorase, and to the respiratory-chain inhibitors, antimycin A and cyanide. The phosphate/oxygen ratio observed for the system is two. The reaction is specific with regard to the quinone; closely related naphthoquinones, such as 1,4-naphthoquinone, as well as various benzoquinones, are inactive. The possible significance of DT diaphorase in the regulation of the cellular levels of the extramitochondrial reduced pyridine nucleotides is discussed.
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