DT-13, a steroidal saponin from Liriope muscari L. H. Bailey, suppresses A549 cells adhesion and invasion by inhibiting MMP-2/9

Abstract

AimTo present the anti-tumor metastatic activity of the saponin derivative DT-13 on the A549 cell line. MethodsThe effective concentration of DT-13 was determined by the MTT assay; the ability of DT-13 on anti-adhesion was detected with the assistance of HUVECs and fibronectin; the ability of DT-13 on anti-invasion was detected by the transwell plate method; the expression of MMP-2 and MMP-9 was determined by Western blotting. Results and ConclusionsDT-13 could inhibit the proliferation and adhesion of A549 cells to HUVECs and fibronectin, and the invasion of A549 cells through the extracellular matrix. In addition, DT-13 could inhibit the expression of MMP-2 and MMP-9 in A549 cells.