Abstract
Differential scanning calorimetry (DSC) is applicable to studying the thermal properties of bacteria when treated with heat, cold, or antibiotics. Foodborne pathogens are inactivated by heat, and denaturation transitions observed by DSC indicate potential sites of cellular injury. Ribosomes, which are the sites for messenger RNA translation, are one critical component of thermal damage as evidenced by characteristic denaturation transitions in the 66-74°C range. These transitions disappear when cells of Clostridium perfringens are subjected to heat, suggesting structural or conformational changes to ribosomal proteins, and when cells of Listeria monocytogenes are cold-shocked by refrigeration, indicating ribosomal dissociation. DSC can be used to show that refrigeration followed by heat treatment improves the killing of dangerous microorganisms.
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