Dryopteris crassirhizomaDryocrassinABBAfor Postharvest Control of the Potato Dry Rot PathogenFusarium solanivar. coeruleum

Abstract

AbstractDryopteris crassirhizomadryocrassinABBAtreatment was tested for its effectiveness in controllingFusarium solanivar. coeruleumgrowthin vitroand for prevention of postharvest dry rot of potato tubers and slices. DryocrassinABBAstrongly inhibited mycelial growth, resulting in reductions in both mycelium dry weight and per cent spore germination ofF. solanivar.coeruleumat concentrations of 2.0, 0.5 and 0.1 mg/ml. Scanning electron microscopy (SEM) observations showed that treatment induced abnormal, tightly twisted morphological changes in hyphae. Moreover,in vivoexperiments demonstrated that dryocrassinABBAtreatment at 2 mg/mleffectively controlled dry rot of potato tubers inoculated with mycelial discs ofF. solanivar.coeruleum. After dryocrassinABBAtreatment, the content of soluble proteins, peroxidase (POD) and superoxide dismutase (SOD) activity increased, and malondialdehyde (MDA) content remained the stable situation. In addition, the expression level of plant lipid‐transfer proteins (LTPs) genes –StLTPa1,StLTPa7,StLTPb1andStLTPb3 –was upregulated. These results collectively demonstrate that dryocrassinABBAcan inhibit growth ofFusariumpathogens to induce disease resistance. On the other side, dryocrassinABBAmaybe induce potato defence responses.