Drying method comparison of black mangrove leaves (Rhizophora mucronata) for an antioxidant activity assay

Abstract

Ethanolic extracts of black mangrove leaf (Rhizophora mucronata) can be a natural source of antioxidants. The phenolic content can affect the activity of antioxidants in the sample. This study aimed to determine black mangrove leaf (Rhizophora mucronata) chemical content primarily focused on total phenolic content and antioxidant activity during sun and wind drying using DPPH, ABTS and CUPRAC analysis methods. Ethanolic extracts were obtained from the maceration method with 1:5 sample and solvent ratio. The total phenolic content value was 1691.2 mg GAE/g with an extract yield of 12.57%. The antioxidant activity of DPPH, ABTS, and CUPRAC method values were 16.61 ppm, 11.07 ppm and 103.52 mg ascorbic acid/g extract and classified as a very strong antioxidant. The best result showed that the highest content of phenolic content and antioxidant activity could be found in the wind-dried extract. The result indicated that ethanolic extract of Rhizophora mucronata leaves can be utilized as a strong antioxidant agent. Drying methods can be used as a good way to preserve mangrove leaves while maintaining their antioxidant activity.