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Abstract
The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null- transfected HepG2 cell lines. The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.
Highlights
It had been reported by IARC that cancer has caused great threat to the people’s health, regardless the state of economy
Real-time RT-PCR In order to analysis the amplification fold of ribosomal protein L24 (RPL24) gene, real-time quantitative RT-PCR was used in drugresistant human hepatocellular carcinoma HepG2 cell line and in susceptible HepG2 cell line
In order to determine the relative copy numbers, the cycle number of RPL24 was normalized against β-actin cycle number by which the amplification reached the threshold between drugresistant human hepatocellular carcinoma HepG2 cell line and susceptible HepG2 cell line
Summary
It had been reported by IARC that cancer has caused great threat to the people’s health, regardless the state of economy. Previous studies of our research group revealed that the hepatocellular carcinoma cell HepG2 adriamycin resistant strain has a high expression level of a series of ribosomal proteins, such as ribosomal protein L24 (RPL24). The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells
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