Abstract
The sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) is an intracellular membrane transporter that utilizes the free energy provided by ATP hydrolysis for active transport of Ca2+ ions from the cytoplasm to the lumen of sarco(endo)plasmic reticulum. SERCA plays a fundamental role for cell calcium homeostasis and signaling in muscle cells and also in cells of other tissues. Because of its prominent role in many physiological processes, SERCA dysfunction is associated to diseases displaying various degrees of severity. SERCA transport activity can be inhibited by a variety of compounds with different chemical structures. Specific SERCA inhibitors were identified which have been instrumental in studies of the SERCA catalytic and transport mechanism. It has been proposed that SERCA inhibition may represent a novel therapeutic strategy to cure certain diseases by targeting SERCA activity in pathogens, parasites and cancer cells. Recently, novel small molecules have been developed that are able to stimulate SERCA activity. Such SERCA activators may also offer an innovative and promising therapeutic approach to treat diseases, such as heart failure, diabetes and metabolic disorders. In the present review the effects of pharmacologically relevant compounds on SERCA transport activity are presented. In particular, we will discuss the interaction of SERCA with specific inhibitors and activators that are potential therapeutic agents for different diseases.
Highlights
P-type ATPases are membrane transporters that couple the energy provided by ATP hydrolysis to the active transport of various ions or phospholipids
sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) plays an essential role in the maintenance of cellular calcium homeostasis and calcium pump malfunction is associated to severe disorders and several pathophysiological conditions
The SERCA enzyme represents an important target for the development of new drugs
Summary
P-type ATPases are membrane transporters that couple the energy provided by ATP hydrolysis to the active transport of various ions or phospholipids. To investigate the effects of pharmacologically relevant compounds on SERCA transport activity, we employ an electrophysiological technique, which is discussed . SSM-based electrophysiology was successfully employed to investigate drug interactions with P-type ATPases.
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