Drug-drug interactions of amiodarone on simvastatin metabolism in human liver microsomes

Abstract

Objective To investigate the drug–drug interactions of amiodarone (AMD) on simvastatin (SV) in human liver microsomes and the possible underlying mechanisms. Design and methods Time-, Reduced form of nicotinamide–adenine dinucleotide phosphate (NADPH)- and concentration-dependent inhibitions were tested in human liver microsomes (HLM). The logarithm of relative inhibition values were plotted versus preincubation time (0, 5, 10, 15, 20min) for a series concentration of AMD used (0, 2, 5, 25, 50μM), and the slopes determined by linear regression.