Drug delivery without nanoparticle uptake: delivery by a kiss-and-run mechanism on the cell membrane

Daniel Hofmann,Claudia Messerschmidt,Markus B Bannwarth,Volker Mailänder,Katharina Landfester

doi:10.1039/c3cc48130a

Abstract

Nearly all concepts of nanocarriers as drug delivery devices rely on intracellular uptake. Instead, we demonstrate an alternative concept for rapid and specific delivery of cargo by nanoparticles to TIP47+/ADRP+ lipid droplets. The model can serve as a novel strategy for the non-invasive delivery of drugs by releasing hydrophobic cargo, in our case a model dye, through a kiss-and-run mechanism between nanoparticles and the cell membrane.

Highlights

Most concepts of nanocarriers as drug delivery devices rely on intracellular uptake
We demonstrate an alternative concept for rapid and specific delivery of cargo by nanoparticles to tailinteracting protein 47 (TIP47)+/ adipose differentiation-related protein (ADRP)+ lipid droplets
The model can serve as a novel strategy for the non-invasive delivery of drugs by releasing hydrophobic cargo, in our case a model dye, through a kiss-and-run mechanism between nanoparticles and the cell membrane

Summary

Introduction

Most concepts of nanocarriers as drug delivery devices rely on intracellular uptake. The model can serve as a novel strategy for the non-invasive delivery of drugs by releasing hydrophobic cargo, in our case a model dye, through a kiss-and-run mechanism between nanoparticles and the cell membrane. Nanoparticles briefly interact with the lipid bilayer of a cell (A) or a giant unilamellar vesicle (B), thereby releasing its dye that accumulates in lipid droplets (LD) or in the membrane, respectively.

Results

Conclusion