Drug Deliverable, Self-assembled Rosette Nanotubes (RNTs) for Orthopedic Applications

Abstract

AbstractRosette nanotubes (RNTs) are novel, biomimetic, synthetic, self-assembled drug delivery agents. Because of base stacking and hydrophobic interactions, the RNT hollow-tube structure can be used for incorporating drugs. Another advantage of using RNTs is their ability to be injected and become solid at body temperatures for orthopedic applications without the use of any external stimuli (such as UV light or crosslinking agents). The nano-features of RNTs create a favorable, biologically-inspired, cellular environment. In this study, methods to incorporate dexamethasone (DEX, a bone growth promoting drug) into RNTs were investigated. The drug-loaded RNTs were characterized using Nuclear Magnetic Resonance (NMR), Diffusion Ordered Spectroscopy (DOSY) and Ultraviolet-visible Spectroscopy (UV-vis). Results showed that small molecular drugs with hydrophobic aromatic rings were incorporated into RNTs. Subsequent drug release experiments demonstrated that DEX was released from the RNTs and had a positive impact on osteoblast functions. Importantly, compared to other drug carriers, RNTs increased the total drug loading and was the highest when DEX was incorporated during the RNT self-assembly process. Thus, this study offered a novel drug delivery device that itself is bioactive and can be used to deliver a variety of drugs for various orthopedic applications.