Drug-Coated Floss to Treat Gum Diseases: In Vitro and In Vivo Characterization.

Abstract

Treatment of gum disease often requires antibiotic treatment. In this study, our objective was to advance the practicality of drug-coated floss as an intra gum pocket drug delivery system. The initial design of this delivery system has been previously reported by us. Here, we advance the concept further through in vitro and in vivo evaluation. A floss piece was dip coated in the middle section with model molecules leaving free ends for holding. Porcine gum tissues were used ex vivo and in vivo to evaluate the coated floss, including effect of coating thickness on delivery efficiency, ability to deliver more than one type of molecule (one hydrophilic and one hydrophobic), mechanical properties using a scratch test, and finally retention of delivered material in vivo in the porcine model. After reaching a certain coating thickness, the delivery efficiency of the coated floss decreased, indicating the presence of an optimal coating thickness. Hydrophobic and hydrophilic molecules were successfully coated and delivered with high efficiency into gum pockets. The scratch test indicated that the coatings were resilient. Lastly, the in vivo analysis showed that the drug coating was delivered into the porcine gum pocket with about 65% efficiency, and the coatings could maintain extended residency within the gum pocket despite the native adverse environment of the oral cavity. Overall, this data shows that drug-coated floss can act as a drug delivery vehicle and has potential to provide a minimally invasive and practical method for the delivery of drugs into the gum pockets.