Abstract
Because drought is a major environmental factor that causes serious agricultural problems, understanding the mechanisms and genetic bases underlying plant responses to drought stress is essential. Using the Brassica rapa 135 K microarray, BrDST71 gene was identified. BrDST71 expression in drought-tolerant Chinese cabbage showed an eight-fold decrease than in wild-type, and encodes a 362 amino acid protein containing a secretory peroxidase domain. Overexpression and RNAi vectors of BrDST71 were constructed and each vector was transformed into Nicotiana tabacum by the Agrobacterium-mediated transformation method. The expression level of BrDST71 and the phenotype were analyzed under drought condition. Transgenic lines with suppressed expression of BrDST71 showed more tolerance to drought stress compared to wild-type and overexpression transgenic lines. It showed that suppressing BrDST71 expression is correlated to better growth under drought conditions. Based on these results, we suggest that down-regulation of BrDST71 improves drought tolerance.
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