Abstract
Drought stress adversely affects plant productivity. Growth and timber production of Paulownia trees are limited under drought stress. Changes in gene expression patterns and miRNA in different ploidy of Paulownia tomentosa have been investigated. However, the responses of P. tomentosa to drought stress at the microRNA (miRNA) level have not been reported so far. To identify miRNA candidates and their target genes involved in the drought stress response in diploid and tetraploid P. tomentosa, four small RNA and four degradome libraries from diploid and autotetraploid P. tomentosa under normal and drought stress conditions were constructed and sequenced. A total of 41 conserved and 90 novel miRNAs were identified. Among these miRNAs, 67 (26 conserved and 41 novel) and 53 (six conserved and 47 novel) were significantly differentially expressed in response to drought stress in diploid and autotetraploid P. tomentosa, respectively. Degradome analysis identified 356 candidate miRNA target genes that encoded proteins with functions that included plant defense, transcriptional regulation, and hormone metabolism. In particular, miR4 and miR156 were identified only in autotetraploid P. tomentosa under drought stress. These results will help us build a foundation for future studies of the biological functions of miRNA-mediated gene regulation in P. tomentosa.
Highlights
Paulownia is a genus of fast-growing deciduous tree species in the family Paulowniaceae native to China
To identify miRNA candidates and their target genes involved in the drought stress response in diploid and tetraploid P. tomentosa, four small RNA and four degradome libraries from diploid and autotetraploid P. tomentosa under normal and drought stress conditions were constructed and sequenced
Diploid and autotetraploid P. tomentosa tissue culture seedlings were first cultured for 30 days and the plants were transferred into nutrition blocks containing ordinary garden soil
Summary
Paulownia is a genus of fast-growing deciduous tree species in the family Paulowniaceae native to China. Paulownia trees are characterized by high biomass production and lightweight strong wood. Because the Paulownia root system can grow in deep soil, Paulownia is considered to be an eco-friendly multi-purpose species for use in agroforestry. Paulownia tree species have been successfully used in intercropping, and the plantation area was estimated to be over two million hectares (Lu 2006). Paulownia has become important to the Chinese economy, and is a focus of research. Drought is recognized as an important factor that limits the growth and production of Paulownia. With the possible effects of global warming becoming a focus of attention, improving the tolerance of plants to drought has become a primary objective in plant breeding.
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