Drought stress tolerance of two wheat genotypes

Abstract

Biotic and abiotic stress effects can limit the productivity of plants to great extent. In Hungary, drought is one of the most important constrains of biomass production, even at the present climatic conditions. The climate change scenarios, developed for the Carpathian basin for the nearest future predict further decrease in surface water resources. Consequently, it is essential to develop drought stress tolerant wheat genotypes to ensure sustainable and productive wheat production under changed climate conditions. The aim of the present study was to compare the stress tolerance of two winter wheat genotypes at two different scales. Soil water regime and development of plants, grown in a pot experiment and in large undisturbed soil columns were evaluated. The pot experiments were carried out in a climatic room in three replicates. GK Élet wheat genotype was planted in six, and Mv Emese in other six pots. Two pots were left without plant for evaporation studies. Based on the mass of the soil columns without plant the evaporation from the bare soil surface was calculated in order to distinguish the evaporation and the transpiration with appropriate precision. A complex stress diagnosis system was developed to monitor the water balance elements. ECH<sub>2</sub>O type capacitive soil moisture probes were installed in each of the pots to perform soil water content measurements four times a day. The irrigation demand was determined according to the hydrolimits, derived from soil hydrophysical properties. In case of both genotypes three plants were provided with the optimum water supply, while the other three ones were drought-stressed. In the undisturbed soil columns, the same wheat genotypes were sawn in one replicate. Similar watering strategy was applied. TDR soil moisture probes were installed in the soil at various depths to monitor changes in soil water content. In order to study the drought stress reaction of the wheat plants, microsensors of 1.6 mm diameter were implanted into the stems and connected to a quadrupole mass spectrometer for gas analysis. The stress status was indicated in the plants grown on partly non-irrigated soil columns by the lower CO<sub>2</sub> level at both genotypes. It was concluded that the developed stress diagnosis system could be used for soil water balance elements calculations. This enables more precise estimation of plant water consumption in order to evaluate the drought sensitivity of different wheat genotypes.