Abstract
Drought threatens the world’s food production, particularly in Sub Saharan Africa low external input and rain fed agricultural systems, where cowpea (Vigna unguiculata (L.) Walp.) is an important food crop. In the context of growing concerns regarding climate changes implications on water availability, this study aimed at 1) to evaluate the drought responses in cowpea landraces with contrasting drought tolerance levels (A55 – high sensitivity; A80 - mild sensitivity; A116 - tolerant), 2) using an integrated physiological (leaf gas exchanges; chlorophyll a fluorescence) and biochemical (photoprotective pigments; RuBisCO activity; primary metabolite profiling) analysis to identify drought tolerance probes, in plants submitted to three water availability levels (well-watered, WW; mild drought, MD; severe drought, SD). A116 plants maintained a better water status under drought, what could justify the higher Pn and Pnmax values in MD, as well as higher photochemical use of energy (reflected in the photochemical quenching (qL) and in the quantum yield of non-cyclic electron transport (Y(II))), and the lower need of photoprotective thermal dissipation mechanisms (given by the non-photochemical quenching (qN), and the quantum yield of regulated energy dissipation at photosystem PSII (Y(NPQ))), in MD and SD plants. Greater declines of net (Pn) and potential (Pnmax) photosynthesis were observed in A55 plants, which frequently showed significant impacts already under MD conditions in most parameters, whereas A80 usually displayed and intermediate behaviour. Still, even A55 showed some acclimation response, regarding photoprotective mechanisms associated with high contents of zeaxanthin, lutein, and carotenes, and high Y(NPQ), and qN values, supporting the absence of an increase in the non-regulated energy dissipation at PSII (Y(NO) did not increased) even in SD plants. Additionally, A55 was not significantly affected in RuBisCO activity, which showed to be quite resilient in cowpea. A primary metabolite profiling, complemented with a partial least square discrimination analysis (PLS-DA), allowed a better separation of A116 and A55 plants according to their degree of drought tolerance. In response to drought, A116 showed the greatest accumulation of most responsive metabolites, 14 in total, with sucrose, fucose, urea, alanine and putrescine being exclusively increased in this genotype, suggesting that they can be candidates as drought tolerance proxies. Other compounds, as proline, valine, isoleucine (among amino acids), and rhamnose and raffinose (among sugars) showed close increase patterns across landraces, thus they would be involved in the common drought response of cowpea plants. This pioneer approach to characterize Africa’s and Central Mozambique highly diverse cowpea germplasm, highlights the advantages of an integrated physiological and biochemical analysis, to identify response mechanisms and potential probes for drought tolerance in cowpea, aiming at to support selection and breeding strategies to obtain high yield and drought tolerant elite varieties.
Highlights
World population is projected to reach ca. 9.800 million around 2050 (FAO - Food and Agriculture Organization of the United Nations (2009); FAO - Food and Agriculture Organization of the United Nations, 2011), bringing along a high pressure on land and water resources for food and feed production (FAO - Food and Agriculture Organization of the United Nations, 2016; Tian et al, 2016; IFPRI - International Food Policy Research Institute, 2019; Pais et al, 2020)
The present study investigated the impact of mild (MD) and severe (SD) water restriction levels in three cowpea landraces with contrasting drought tolerance (A55 – high sensitivity; A80 – mild sensitivity; A116 - tolerant), from three agro-ecological zones of Central Mozambique
Kinetic traits can vary among RuBisCO forms, likely reflecting different environmental selective pressures during evolution (Iñiguez et al, 2020), and improvements in their overall catalytic performance through biotechnological approaches was suggested to constitute a way to significantly increase plant yield, especially under stressful environmental conditions (Parry et al, 2013; Lin et al, 2014)
Summary
World population is projected to reach ca. 9.800 million around 2050 (FAO - Food and Agriculture Organization of the United Nations (2009); FAO - Food and Agriculture Organization of the United Nations, 2011), bringing along a high pressure on land and water resources for food and feed production (FAO - Food and Agriculture Organization of the United Nations, 2016; Tian et al, 2016; IFPRI - International Food Policy Research Institute, 2019; Pais et al, 2020). 9.800 million around 2050 (FAO - Food and Agriculture Organization of the United Nations (2009); FAO - Food and Agriculture Organization of the United Nations, 2011), bringing along a high pressure on land and water resources for food and feed production (FAO - Food and Agriculture Organization of the United Nations, 2016; Tian et al, 2016; IFPRI - International Food Policy Research Institute, 2019; Pais et al, 2020) This will constitute an enormous challenge, in Sub-Saharan Africa, where the population is estimated to double by 2050 (UN -United Nations, 2019), and are expected strong impacts of climate changes on the predominant rain fed and low external input agricultural systems (Mendelsohn, 2008; Müller et al, 2011). In plants with a higher acclimation ability, such limited photochemical energy use can thereafter trigger, among others, thermal dissipation and photoprotective mechanisms, cyclic electron flow, and increase the presence of antioxidative molecules (e.g., enzymes, carotenoids) (Chaves and Oliveira, 2004; Reddy et al, 2004; Ramalho et al, 2018)
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