Drought‐induced spikelet sterility is associated with an inefficient antioxidant defence in rice panicles

Abstract

Water stress‐induced spikelet sterility limits rice production under upland conditions. The causes of spikelet sterility under drought stress are poorly understood. In this study the role of antioxidant defence management in drought‐induced spikelet sterility was investigated in two rice (Oryza sativa) genotypes differing in drought resistance. Drought‐resistant N22 genotype showed less water stress‐induced spikelet sterility when compared to the susceptible N118 genotype under upland conditions. The N22 panicles maintained higher RWC and turgor potential and lower H2O2 levels across the developmental stages under water stress than that of N118 panicles. Drought‐induced enhancement in superoxide dismutase (SOD, EC 1.15.1.1) activity coupled with higher ascorbate (AsA), glutathione (GSH) content and enhanced ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) activities resulted in lower H2O2 levels in N22 panicles. In contrast, insufficient enhancement in SOD, APX and GR activities resulted in relatively higher H2O2 levels under water stress in N118 panicles. The N22 panicles exhibited a higher number of SOD and APX isozymes in comparison with N118 panicles that might provide better reactive oxygen species scavenging. Hence it is concluded that well‐equipped antioxidant defence plays an important role in minimizing water stress‐induced spikelet sterility in upland rice.