Abstract
During central nervous system (CNS ) development, a large number of neurons die by apoptosis and are efficiently removed through phagocytosis. Since apoptosis and apoptotic cell clearance are highly conserved in evolution, relatively simple and easily accessible Drosophila embryonic CNS provides a good model to study molecular and cellular mechanisms of these processes. Here, we describe how to assess neuronal apoptosis and glial phagocytosis of apoptotic neurons using immunohistochemistry of whole fixed embryos and live imaging of developing embryonic CNS. Combination of these different strategies allows a comprehensive analysis of neuronal cell death in vivo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
