Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Abdullah I A Al-Mubarak,Faisal Almathen,Naser Abdallah Al Humam,Turke Shawaf,Hans-Joachim Schuberth,Jamal Hussen

doi:10.1186/s12917-020-02285-8

Abdullah I A Al-Mubarak, Faisal Almathen + Show 4 more

Open Access

https://doi.org/10.1186/s12917-020-02285-8

Copy DOI

Abstract

BackgroundIn human and different animal species, blood monocytes are classified based on their expression pattern of different monocytic markers into phenotypically and functionally different subsets. In the current study, we used flow cytometry and monoclonal antibodies to CD172a, CD14, CD163 and MHCII to identify monocyte subsets in peripheral blood of dromedary camels.ResultsBased on CD14, CD163 and MHCII expression, camel CD172a + monocytes were divided into three subsets: The major subpopulation of camel monocytes (mo-I) showed high expression of CD14 and CD163, but low expression of MHCII. A second subset of monocytes (mo-II) expressed highly all three markers, CD14, CD163 and MHCII. A third monocyte subset (mo-III) displayed low expression of CD14 and CD163 with high MHCII expression. While the two MHCIIhigh subsets (mo-II and mo-III) showed higher expression of CD11a in comparison to the MHCIIlow subset (mo-I), CD18 and CD11b were highest expressed on the two CD14high subsets (mo-I and mo-II). Bacterial stimulation of camel leukocytes identified mo-II cells as an antimicrobial monocyte subset with the highest phagocytic and ROS production capacity. The comparison of monocyte counts and phenotype between newborn calves and adult camels revealed significantly reduced numbers of mo-II cells in newborn animals. Monocytes of newborns expressed significantly more CD172a and CD163 molecules but less CD14 and MHCII molecules than monocytes of adult camels.ConclusionsCamel monocyte subsets, mo-I, mo-II and mo-III are counterparts of bovine classical, intermediate and non-classical monocytes respectively. The distribution of camel monocyte subsets is influenced by age.

Highlights

In human and different animal species, blood monocytes are classified based on their expression pattern of different monocytic markers into phenotypically and functionally different subsets
Based on the cell surface expression of CD14 and MHCII, three monocyte subsets were defined after flow cytometry (Fig. 1c)
A third monocyte subset displayed low expression of CD14 and CD163 with high MHCII expression (CD14lowCD163lowMHCIIhigh) (Fig. 2), which was higher than MHCII expression on mo-I but lower than MHCII expression on mo-II (Fig. 2b)

Summary

Introduction

In human and different animal species, blood monocytes are classified based on their expression pattern of different monocytic markers into phenotypically and functionally different subsets. Studies in humans [4], mice [5], cows [6,7,8], pigs [9] and dogs [10] have classified monocytes into different subsets with subset-specific phenotype and function [11]. Hussen et al BMC Veterinary Research (2020) 16:62 monocyte subsets based on CD14 and MHCII molecules expression patterns [10]. Monocyte subsets showed phenotypic and functional differences in a species-specific manner. This includes the expression of different monocytic markers, cell adhesion molecules, cytokines and chemokine receptors [11, 15]. Human and bovine CD14high monocytes, including classical and intermediate monocytes, showed enhanced anti-bacterial activity, including phagocytosis and ROS generation capacity [6, 12], whereas a patrolling function along the endothelium and a role in anti-viral immunity have been described for human and mouse CD14low monocytes (non-classical monocytes) [16]

Objectives

Methods

Results

Conclusion