Drivers of bacterial colonization patterns in stream biofilms

Abstract

Dispersal and colonization are important for the assembly and biodiversity of microbial communities. While emigration as the initial step of dispersal has become increasingly understood in model bacterial biofilms, the drivers of dispersal and colonization in complex biofilms remain elusive. We grew complex biofilms in microcosms from natural surface water in laminar and turbulent flow, and investigated dispersal and colonization patterns of fluorescently labeled cells and microbeads in nascent and mature biofilms. Settling occurred in nonrandom spatial patterns governed by the interplay of local flow patterns and biofilm topography. Settling was higher in treatments with nascent biofilms, with fewer cells remaining in the water column than in treatments with mature biofilms. The flow regime had no effect on settling velocity, even though in mature biofilms the formation of streamers under turbulent flow enhanced particle trapping compared with the laminar flow treatment. Hence, small-scale variations in the flow pattern seemed to be more important than the overall flow regime. Furthermore, spatial analysis of the colonizer patterns suggests that bacteria have moved in the biofilm after settling. Our results show that colonization of biofilms in a model stream environment is a heterogeneous process differently affected by biological and physical factors.