Drinking Molecular Hydrogen Water Is Beneficial to Cardiovascular Function in Diet-Induced Obesity Mice.

Haruchika Masuda,Atsuko Sato,Kazuo Ishiwata,Yoshihiro Nakagawa,Takayuki Asahara,Tomoko Shizuno,Akira Oyamada,Kumiko Miyata

doi:10.3390/biology10050364

Abstract

Simple SummaryMolecular hydrogen (MH) reportedly exerts therapeutic effects against inflammatory diseases by alleviating oxidative stress. We investigated the cardiovascular protective effects of molecular hydrogen water (MHW) intake using high-fat diet-induced obesity (DIO) mice. We observed that MHW intake for 2 weeks did not improve the blood sugar level or body weight but decreased heart weight in DIO mice. Notably, MHW intake alleviated oxidative stress in both the heart and the adipose tissue. Moreover, it improved cardiac hypertrophy and restored left ventricular function in DIO mice, and promoted the histological conversion of energy storage to expenditure in adipose tissues with the upregulation of thermogenic and cardiovascular protective genes. Furthermore, MHW restored endothelial progenitor cell (EPC) bioactivity to maintain vascular homeostasis. Taken together, MHW intake exerts cardiovascular protective effects in DIO mice. Hence, MHW intake is a potential prophylactic strategy against cardiovascular disorders in metabolic syndrome.Molecular hydrogen (MH) reportedly exerts therapeutic effects against inflammatory diseases as a suppressor of free radical chain reactions. Here, the cardiovascular protective effects of the intake of molecular hydrogen water (MHW) were investigated using high-fat diet-induced obesity (DIO) mice. MHW was prepared using supplier sticks and degassed water as control. MHW intake for 2 weeks did not improve blood sugar or body weight but decreased heart weight in DIO mice. Moreover, MHW intake improved cardiac hypertrophy, shortened the width of cardiomyocytes, dilated the capillaries and arterioles, activated myocardial eNOS-Ser-1177 phosphorylation, and restored left ventricular function in DIO mice. MHW intake promoted the histological conversion of hypertrophy to hyperplasia in white and brown adipose tissues (WAT and BAT) with the upregulation of thermogenic and cardiovascular protective genes in BAT (i.e., Ucp-1, Vegf-a, and eNos). Furthermore, the results of a colony formation assay of bone-marrow-derived endothelial progenitor cells (EPCs) indicated that MHW activated the expansion, differentiation, and mobilization of EPCs to maintain vascular homeostasis. These findings indicate that the intake of MHW exerts cardiovascular protective effects in DIO mice. Hence, drinking MHW is a potential prophylactic strategy against cardiovascular disorders in metabolic syndrome.

Highlights

Molecular hydrogen (MH), the lightest and most abundant chemical element, can act as an antioxidant by suppressing free radical chain reactions, thereby reducing the production of reactive oxygen species (ROS) [1,2,3,4]
MH water (MHW) was transferred to a 0.2 L plastic bottle to degas H2 from MHW for DGW, and the glass bottle the24sticks was refilled with sterilized water forand
This study aimed to determine whether MHW intake can alleviate cardiovascular dysfunction in obesity associated with metabolic syndrome (MetS) in diet-induced obesity (DIO) mice

Summary

Introduction

Molecular hydrogen (MH), the lightest and most abundant chemical element, can act as an antioxidant by suppressing free radical chain reactions, thereby reducing the production of reactive oxygen species (ROS) [1,2,3,4]. The emerging evidence of MH as an antioxidant with the use of clinical and experimental animal models proves that administration of MH, either through hydrogen gas inhalation or drinking water, is a feasible strategy for the treatment of a variety of inflammatory diseases. In animal models, MH gas inhalation and intake of MH water (MHW) were demonstrated to play a significant role in the prevention of various acute and chronic inflammatory diseases, including focal brain ischemia/reperfusion injury [1], myocardial ischemia [5], type 1 and 2 diabetes and insulin resistance [6,7], atherosclerosis [8], liver fibrosis [9], and hypertension [10]. MH intake was reported to be effective against metabolic syndrome (MetS) [12] and the adverse effects of chemotherapy [13] and radiotherapy [14]

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