Dried Blood Spots on Carboxymethyl Cellulose Sheets: Rapid Sample Preparation Based on Dissolution and Precipitation

Abstract

This short communication describes the use of carboxymethyl cellulose sheets as sampling material for dried blood spots. Whole blood, spiked with quetiapine, a hydrophobic and basic small molecule drug substance, was spotted on the sheet and subsequently dried. The dried spot was then almost completely dissolved in acidified aqueous solution. It was shown that the dissolved polymer, together with major blood components can easily be precipitated and removed with acetonitrile. The presented sampling on a water-soluble biopolymer derivative followed by precipitation resulted in a simple protocol and around 100 % recovery of the analyte prior to liquid chromatography—tandem mass spectrometry.