Abstract
Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.
Highlights
The use of Dried blood sample (DBS) in clinical analysis
Our approach involves the absorption of a liquid capillary blood sample onto a precisely shaped strip of Whatman filter paper, wherein the volume absorbed is controlled by the properties and size of the strip
We show here that our volumetric sampling device is capable of collecting an accurate, reproducible volume of blood that can be used for quantitative analysis of DBSs
Summary
The use of DBSs in clinical analysis. the current COVID-19 global pandemic has rapidly increased the interest in at-home sample collection and testing, both for convenience and to minimize risk of infection, and one of the easiest at-home blood sampling techniques is DBS collection. We aim to (1) provide a thorough procedure validation using a volumetric DBS collection device with an established quantitative proteomics analysis method and (2) analyze 1000 proteins using this approach in DBSs and validate measurable proteins for future consideration of utility in clinical applications. To accomplish these aims, we developed a novel volumetric sampling device to collect a precise volume of blood to overcome the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection. Our data provides evidence of the applicability of DBSs for widespread use in clinical applications
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