Abstract
BackgroundAnalyses of replicates in sets of discrete data, typically acquired in multi-well plate formats, is a recurring task in many contemporary areas in the Life Sciences. The availability of accessible cross-platform data analysis tools for such fundamental tasks in varied projects and environments is an important prerequisite to ensuring a reliable and timely turnaround as well as to provide practical analytical tools for student training.ResultsWe have developed an easy-to-use, interactive software tool for the analysis of multiple data sets comprising replicates of discrete bivariate data points. For each dataset, the software identifies the replicate data points from a defined matrix layout and calculates their means and standard errors. The averaged values are then automatically fitted using either a linear or a logistic dose response function.ConclusionsDRfit is a practical and convenient tool for the analysis of one or multiple sets of discrete data points acquired as replicates from multi-well plate assays. The design of the graphical user interface and the built-in analysis features make it a flexible and useful tool for a wide range of different assays.
Highlights
Analyses of replicates in sets of discrete data, typically acquired in multi-well plate formats, is a recurring task in many contemporary areas in the Life Sciences
The majority of them have been designed for particular tasks, such as the analysis of single-cell migration [1] or differential scanning fluorimetry data [2]
The application BiAnaCa was reported as a tool for general biochemical assays [3], but does not appear to be available for download
Summary
Application example: dose response assays Many studies are concerned with dose response or receptor-ligand binding assays. In particular in drug discovery applications, increasing concentrations of small-molecule compounds are tested in protein-based or whole organism assays. Most frequently, such data are analysed using a four-parameter logistic equation that results in a sigmoid curve, in which the point of inflection yields the widely used parameter IC50/EC50. In our ongoing efforts to ‘streamline’ data analysis for this motility assay, DRfit has been successfully deployed, leading to further improvements of time required to conduct screening studies. Application example: enzyme kinetics Most commonly, enzyme kinetics are analysed using the initial rate. Using the linear fit model of DRfit, initial rate analysis of enzyme kinetics data, acquired in a multi-well format, can be carried out conveniently. We have used DRfit to assess the standard (= non burst-like) steady-state kinetics of trehalose-6-phosphate phosphatases [17]
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