Abstract
An idiogram construction following chromosome measurements is a versatile tool for cytological, cytogenetic and phylogenetic studies. The information on chromosome length, centromere index and position of cytogenetic landmarks along with modern techniques (e.g. genomic and fluorescence in situ hybridization, banding, chromosome painting) can help to shed light on genome constitution, chromosome rearrangements and evolution. While idiogram construction is a routine task there are only few freely available programs that can perform chromosome measurements and no software for simultaneous measuring of chromosome parameters, chromosomal landmark and FISH signal positions and idiogram construction. To fill this gap, we developed DRAWID (DRAWing IDiogram), java-based cross-platforming program for chromosome analysis and idiogram construction. DRAWID has number of advantages including a user-friendly interactive interface, possibility for simultaneous chromosome and FISH/GISH/banding signal measurement and idiogram drawing as well as number of useful functions facilitating the procedure of chromosome analysis. The output of the program is Microsoft XL table and publish-ready idiogram picture. DRAWID and the manual for its use are freely available on the website at: http://www.drawid.xyz
Highlights
Chromosome number, morphology and organization are important parameters for comparative cytogenetic and phylogenetic studies (Mandáková and Lysak 2008; Peruzzi et al 2009; Cheng et al 2013; Kirov et al 2014; Divashuk et al 2014; Bolsheva et al 2015; Astuti et al 2017)
Once idiogram adjustments are performed, the idiogram can be added to the DRAWID storage to use it later again
Building an idiogram for cytogenetically uncharacterized species requires an average idiogram based on measurements of several metaphases
Summary
Chromosome number, morphology and organization are important parameters for comparative cytogenetic and phylogenetic studies (Mandáková and Lysak 2008; Peruzzi et al 2009; Cheng et al 2013; Kirov et al 2014; Divashuk et al 2014; Bolsheva et al 2015; Astuti et al 2017). Differences in chromosome morphology between individual species are the result of inter- and intra-chromosomal rearrangements which are major forces of evolution and speciation (Rieseberg 2001; De Storme and Mason 2014; Mandáková et al 2015). Knowledge about chromosome rearrangements and basic chromosome characteristics (e.g. centromere index, arm ratio, relative length, chromosomal asymmetry) can be useful for the integration of physical and genetic maps, the study of speciation and evolution and for tracing desirable traits during plant breeding processes (Peruzzi and Eroğlu 2013; Budylin et al 2014; Laskowska et al 2015; Astuti et al 2017). Cytology-based ecological studies of genome variability require measurements of chromosomes from a large number of individuals
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
