Abstract
We report the draft assemblies of TPD3 and TPD4, two Xylella fastidiosa subsp. fastidiosa isolates infecting grapevines in Hou-li, Taiwan. TPD3 and TPD4 showed similar characteristics regarding genome size (2,483,503 bp and 2,491,539 bp, respectively), GC content (51.49% and 51.47%, respectively), and number of protein-coding sequences (2,394 and 2,413, respectively).
Highlights
Stock [0.2% phenol red in distilled water, 10 ml], glutamine stock [8.0% {wt/vol}, 50 ml], and bovine serum albumin fraction-five stock [20% {wt/vol}, 15 ml]) medium plates, and isolation of a pure clone was done using a streak plate technique done three times in consecutive petri dishes
A total of 9,914,835 paired fastq reads were used for the TPD3 assembly, and a total of 10,054,122 paired fastq reads were used for the TPD4 assembly
The TPD3 genome (818ϫ sequencing depth of coverage) had a GC content of 51.49%, with a genome length of 2,422,083 bp distributed among 377 contigs ranging in size from 203 bp to 94,897 bp, an N50 of 38,877 kb, and an L50 of 20 kb
Summary
Stock [0.2% phenol red in distilled water, 10 ml], glutamine stock [8.0% {wt/vol}, 50 ml], and bovine serum albumin fraction-five stock [20% {wt/vol}, 15 ml]) medium plates, and isolation of a pure clone was done using a streak plate technique done three times in consecutive petri dishes. The DNA provided for Illumina library preparation was at a concentration of 10.06 ng/l and an A260/A280 purity ratio of 1.55 for TPD3. In the case of TPD4, the DNA concentration was 9.67 ng/l, and the A260/A280 purity ratio was 1.42. Isolates were sequenced using an Illumina HiSeq 2000 platform at the University of California, Berkeley Vincent J.
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