Abstract

Klebsiella pneumoniae is a Gram-negative bacterium that can be found in the environment, as well as on mucosal surfaces of humans and animals. Here, we report the genome sequence of five K. pneumoniae isolates from substrate samples and bird feces collected in the Subantarctic Islands of New Zealand.

Highlights

  • Klebsiella pneumoniae is a Gram-negative bacterium that can be found in the environment, as well as on mucosal surfaces of humans and animals

  • The use of animals and sample collection were completed under permit 39915-FAU from the New Zealand Department of Conservation and Massey University Animal Ethics Committee

  • KG, Germany) was used to extract genome-quality DNA from a single colony cultured on agar, which was sent to New Zealand Genomics Limited (Massey Genome Service, Massey University, Palmerston North, New Zealand)

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Summary

Introduction

Klebsiella pneumoniae is a Gram-negative bacterium that can be found in the environment, as well as on mucosal surfaces of humans and animals. Five K. pneumoniae isolates from cloacal swabs or voided feces from subantarctic skuas (n ϭ 2) and a yellow-eyed penguin (n ϭ 1) and substrate samples (water; n ϭ 2) (using CHROMagar Orientation) were whole-genome sequenced. The NucleoSpin soil kit (Macherey-Nagel, GmbH & Co. KG, Germany) was used to extract genome-quality DNA from a single colony cultured on agar, which was sent to New Zealand Genomics Limited (Massey Genome Service, Massey University, Palmerston North, New Zealand).

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