Abstract
Bacillus cereus is a foodborne pathogen causing emetic and diarrheal-type syndromes. Here, we report the whole-genome sequences of 11 B. cereus food isolates.
Highlights
Eleven B. cereus strains isolated from different food sources (8) were grown overnight (18 h) with shaking (200 rpm) in 10 ml of brain heart infusion (BHI) broth (Becton, Dickinson) at 30°C
Two milliliters of this culture were centrifuged at 13,000 ϫ g to harvest the cells, and the resulting cell pellet was resuspended in 50 mM EDTA
The isolated DNA was sheared to 250- to 350-bp fragments and paired-end sequenced on an Illumina HiSeq 2000 outsourced to BaseClear (Leiden, The Netherlands)
Summary
Eleven B. cereus strains isolated from different food sources (8) were grown overnight (18 h) with shaking (200 rpm) in 10 ml of brain heart infusion (BHI) broth (Becton, Dickinson) at 30°C. Two milliliters of this culture were centrifuged at 13,000 ϫ g to harvest the cells, and the resulting cell pellet was resuspended in 50 mM EDTA (pH 8.0).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
