Abstract
To date, Veillonella tobetsuensis has been known as an oral anaerobe and a facilitator of early-stage oral biofilm development with streptococci. Here, we report the draft genome sequences of 2 strains of V. tobetsuensis first isolated from intraoperative bronchial fluids of elderly patients with pulmonary carcinoma.
Highlights
Hasegawa et al [6] isolated and identified Veillonella isolates as predominant anaerobes by 16S rRNA gene sequences from intraoperative bronchial fluids of patients with pulmonary carcinoma
2,878,222 and 2,452,016 reads were obtained for PAGU 1578 and PAGU 1579, respectively, and they were checked for base quality and de novo assembled using CLC Genomics Workbench v. 11.01 (CLCbio)
The results of BLAST calculation of average nucleotide identity (ANI) values between PAGU 1578 and PAGU 1579 or these two clinical isolates and V. tobetsuensis ATCC BAA-2400T, which is the most closely related species, were 96.73% and 96.16% to 97.05%, respectively. These results suggested that PAGU 1578 and PAGU 1579 were the same species, V. tobetsuensis
Summary
Hasegawa et al [6] isolated and identified Veillonella isolates as predominant anaerobes by 16S rRNA gene sequences from intraoperative bronchial fluids of patients with pulmonary carcinoma. The genomic DNA of these 2 isolates was extracted from 5-day cultures using phenol-chloroform extraction and ethanol precipitation [7]. Further purification was carried out using the Wizard Genomic DNA purification kit (Promega) for highthroughput sequences.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
