Abstract

The thermophilic Moorella sp. strains E308F and E306M were isolated from an acidic hot spring in Japan. Here, we report the draft genome sequences of E308F (3.06 Mbp; G+C content, 54.0%) and E306M (2.99 Mbp; G+C content, 54.4%), to advance the genomic information available on the genus Moorella.

Highlights

  • The thermophilic Moorella sp. strains E308F and E306M were isolated from an acidic hot spring in Japan

  • The isolates were grown on modified hypotonic artificial seawater (hASW) medium

  • Mate pair libraries were prepared from DNA using a Nextera mate pair library preparation kit (Illumina, Inc., San Diego, CA, USA), followed by sequencing with the Illumina MiSeq platform using a v3 reagent kit (2 ϫ 300-bp mate pair reads)

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Summary

Introduction

The thermophilic Moorella sp. strains E308F and E306M were isolated from an acidic hot spring in Japan. The isolates were grown on modified hASW medium. Mate pair libraries were prepared from DNA using a Nextera mate pair library preparation kit (Illumina, Inc., San Diego, CA, USA), followed by sequencing with the Illumina MiSeq platform using a v3 reagent kit (2 ϫ 300-bp mate pair reads). Totals of 4,147,082 and 3,134,908 reads were generated for E308F and E306M, respectively.

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