Abstract
Flavobacterium psychrophilum is a Gram-negative, psychrophilic bacterium within the family Flavobacteriaceae Here, we report the draft genome sequences of three F. psychrophilum strains isolated from skin ulcers of diseased ayu caught by tomozuri angling at three sites in the Kagami River in Japan.
Highlights
Flavobacterium psychrophilum is a Gram-negative, psychrophilic bacterium within the family Flavobacteriaceae
Single colonies of three strains were picked into 10 ml of modified cytophaga (MCYT) broth and grown for 4 days at 18°C
Raw reads were imported into CLC Genomics Workbench 9.5.3 (Qiagen) with default parameters for trimming and removing low-quality base calls and de novo assembly of high-quality reads
Summary
Flavobacterium psychrophilum is a Gram-negative, psychrophilic bacterium within the family Flavobacteriaceae. Single colonies of three strains were picked into 10 ml of MCYT broth and grown for 4 days at 18°C. Following centrifugation at 10,000 ϫ g for 10 min at 4°C, genomic DNA samples were prepared using Genomic-tip 500/G columns and the recommended buffer system (Qiagen) according to the manufacturer’s instructions.
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