Draft Genome Sequences of Three Flavobacterium psychrophilum Strains Isolated from Coldwater Disease Outbreaks at Three Production Hatcheries.

Regg Neiger,Seema Das,Joy Scaria,Kevin Snekvik,Milton Thomas,Brian Fletcher,Michael Barnes,Jim Thompson

doi:10.1128/genomea.00035-16

Abstract

We report here the genome sequences of three Flavobacterium psychrophilum strains causing a bacterial coldwater disease (BCWD) outbreak, isolated from infected rainbow trout from hatcheries in Montana and South Dakota. The availability of these virulent outbreak-causing strain genome sequences will help further understand the pathogenesis of BCWD.

Highlights

For isolating genomic DNA, strains were grown in tryptone yeast extract medium for 5 days at 20°C
DNA from each strain was isolated from 1.0 ml of grown cultures using the E.Z.N.A. bacterial DNA kit (Omega Bio-tek, Norcross, GA)
The genomes were sequenced on an Illumina MiSeq platform using V2 paired-end chemistry (2 ϫ 250 bp)

Summary

Introduction

For isolating genomic DNA, strains were grown in tryptone yeast extract medium for 5 days at 20°C. Bacterial coldwater disease (BCWD) is a disease that affects a number of free-ranging and cultured salmonid and a variety of nonsalmonid fish species [1,2,3,4]. The causative agent of BCWD is the yellow-pigment-producing bacterium Flavobacterium psychrophilum.

Results

Conclusion