Abstract

The draft genomes of three fungal clinical isolates of Madurella mycetomatis from patients with mycetoma are presented. No finished genome is currently available for this important fungus. Therefore, the addition of these new draft genomes will help us better understand the diversity and pathogenicity of this important species.

Highlights

  • Madurella mycetomatis is an ascomycetous dematiaceous fungus responsible for most black-grain eumycetoma worldwide (1, 2)

  • M. mycetomatis strains were isolated by direct culture of the black grains obtained by a deep surgical biopsy and identified by morphology and PCR

  • Sequencing the three isolates resulted in 5,160,000 reads for strain NMM13, 5,480,000 reads for strain NMM18, and 7,020,000 reads for strain NMM15

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Summary

Introduction

Madurella mycetomatis is an ascomycetous dematiaceous fungus responsible for most black-grain eumycetoma worldwide (1, 2). M. mycetomatis clinical isolates were obtained from patients attending the mycetoma clinic at the Mycetoma Research Centre of Khartoum University (Khartoum, Sudan). Primary isolation of M. mycetomatis was performed by culturing washed black grains on Sabouraud’s dextrose agar with chloramphenicol to inhibit bacterial growth, followed by incubation at 37°C for 1 week.

Results
Conclusion
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