Abstract
Chromobacterium subtsugae was isolated from cranberry bogs in Massachusetts. While it is unknown what environmental role these bacteria play in bog soils, they hold potential as biological control agents against the larvae of insect pests. Potential virulence genes were identified, including the violacein synthesis pathway, siderophores, and several chitinases.
Highlights
95.3% homologous by this method, 90 to 96% homologous to the C. subtsugae reference genome, confirming them as a member of this species
Chromobacterium subtsugae strains were isolated from cultivated (MWU3525) and wild (MWU2576, MWU2920) cranberry bog soils in Massachusetts, and tentatively identified as C. subtsugae by phenotype and by 16S rRNA sequences [1,2,3]. Their genomes were sequenced at the University of Arizona Genetics Core using a 454 GS FLX Titanium following manufacturer protocols
Three samples were loaded into each region of a sequencing PTP plate divided with a 2-region gasket
Summary
95.3% homologous by this method, 90 to 96% homologous to the C. subtsugae reference genome, confirming them as a member of this species. Chromobacterium subtsugae strains were isolated from cultivated (MWU3525) and wild (MWU2576, MWU2920) cranberry bog soils in Massachusetts, and tentatively identified as C. subtsugae by phenotype and by 16S rRNA sequences [1,2,3]. Their genomes were sequenced at the University of Arizona Genetics Core using a 454 GS FLX Titanium following manufacturer protocols. The emPCR Amplification Method manual, Lib-L LV, XLϩ (June 2013), was followed for bulk emPCR of each sample.
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