Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs.

Janine T Bossé,Matthew T G Holden,Øystein Angen,Andrew N Rycroft,Lucy A Weinert,Roberto Fernandez Crespo,Yanwen Li,Duncan J Maskell,Paul R Langford,Brendan W Wren,Alexander W Tucker

doi:10.1128/mra.00716-19

Abstract

We report here the draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319). These NAD-dependent bacterial species are frequently found in the upper respiratory tract of pigs and are occasionally associated with lung pathology.

Highlights

We report here the draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319)
Strains 46K2CT and NM319T were obtained from the Culture Collection University of Gothenburg (CCUG 39029T and CCUG 38924T, respectively), stored frozen at Ϫ80°C in 25% glycerol, and minimally passaged on brain heart infusion agar (Difco) supplemented with 0.01% NAD prior to genomic DNA extraction using a FastDNA spin kit (MP Biomedicals), according to the manufacturer’s protocol for bacterial cells
The draft genome of A. indolicus 46K2CT was assembled from 2,031,716 total reads, with an average quality score of 37.9, into 22 contigs (N50, 183,245; L50, 4), with a total length of 2,103,350 bases

Summary

Introduction

We report here the draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319). Strains 46K2CT and NM319T were obtained from the Culture Collection University of Gothenburg (CCUG 39029T and CCUG 38924T, respectively), stored frozen at Ϫ80°C in 25% glycerol, and minimally passaged (up to four times) on brain heart infusion agar (Difco) supplemented with 0.01% NAD prior to genomic DNA extraction using a FastDNA spin kit (MP Biomedicals), according to the manufacturer’s protocol for bacterial cells. Draft genome sequences were assembled de novo using a previously reported pipeline [15].

Results

Conclusion