Abstract
We report here the draft genome sequences of Staphylococcus bacteriophages JBug18, Pike, Pontiff, and Pabna, which infect and lyse S. epidermidis and S. aureus strains. All bacteriophages belong to the morphological family Podoviridae and constitute attractive candidates for use as whole-phage therapeutics due to their compact genomes and lytic lifestyles.
Highlights
We report here the draft genome sequences of Staphylococcus bacteriophages JBug18, Pike, Pontiff, and Pabna, which infect and lyse S. epidermidis and S. aureus strains
Promega Wizard DNA cleanup kit in a modified protocol (8). Their DNA was sequenced in an Illumina MiSeq platform using 2 ϫ 250-bp chemistry at the Genomic Sequencing and Analysis Facility at the University of Texas in Austin, Texas
Reads of JBug[18], Pike, Pontiff, and Pabna were each assembled into a single contig using SPAdes v1.0 with k-mer sizes of 21, 33, and 55
Summary
We report here the draft genome sequences of Staphylococcus bacteriophages JBug18, Pike, Pontiff, and Pabna, which infect and lyse S. epidermidis and S. aureus strains. Purified phages were propagated and their genomes were extracted using a Volume 8 Issue 8 e00054-19 Received 13 January 2019 Accepted 25 January 2019 Published 21 February 2019 mra.asm.org 1 JBug18 - MH972263 - 18,547 nt host: S. epidermidis LM1680
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