Abstract
The chicken is the most common domesticated animal and the most abundant bird in the world. However, the chicken gut is home to many previously uncharacterized bacterial taxa. Here, we report draft genome sequences from six bacterial isolates from chicken ceca, all of which fall outside any named species.
Highlights
We cultured 37 bacterial isolates from post-mortem cecal contents of commercially raised 35-day-old Ross broilers. 16S rRNA gene sequencing suggested that six isolates were distinct from all previously named bacterial species due to placement in a taxonomic tree built with ARB, and so these isolates were selected for whole-genome sequencing (1)
The genomes varied in size from 2.49 Mb/2248 coding sequences (CDSs) for CHCKI005 to 3.99 Mb/3686 CDSs for CHCKI004
An in silico DNA-DNA hybridization analysis of the genomes was completed using GGDC2.0 (6) and average nucleotide identity was performed using JSpecies (7) against the nearest bacterial species to ensure the isolates did not belong to known species. 16S rRNA gene sequences were analyzed using the ARB software package and the LTP_121 database from the All-Species Living-Tree project
Summary
The genomes varied in size from 2.49 Mb/2248 coding sequences (CDSs) for CHCKI005 to 3.99 Mb/3686 CDSs for CHCKI004. 16S rRNA gene sequencing suggested that six isolates were distinct from all previously named bacterial species due to placement in a taxonomic tree built with ARB, and so these isolates were selected for whole-genome sequencing (1). Genomic DNA (1 ng) was prepared using the Nextera XT DNA sample preparation kit (Illumina) followed by sequencing on the Illumina MiSeq platform using the paired-end 2 ϫ 250-bp (version 2) protocol.
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