Abstract
The genome sequences of four Lactococcus lactis strains isolated from fermented camel milk were sequenced using paired-end Illumina MiSeq reads. The genome size of each strain was about 2.6 Mb, and three of the strains were annotated with tet(S) coding for tetracycline resistance.
Highlights
We report the draft genome sequences of L. lactis strains MS22314, MS22333, MS22336, and MS22337
DNA concentrations were measured on the Qubit fluorometer using the doublestranded DNA high-sensitivity (HS) assay kit (Invitrogen)
The pooled Nextera XT libraries were loaded onto an Illumina MiSeq reagent cartridge using the MiSeq reagent kit v3 and 500 cycles with a standard flow cell
Summary
We report the draft genome sequences of L. lactis strains MS22314, MS22333, MS22336, and MS22337. Camel milk samples (n = 29) were collected from several farms in the Babile area of Ethiopia and incubated at 30°C or 42°C for 48 h to stimulate fermentation. Single colonies from 114 isolates on one of the agar plates were chosen for 16S rRNA gene sequencing as described by Fugl et al [2]. Based on phenotypic characterization [2], single colonies from each of four isolates, MS22314, MS22333, MS22336, and MS22337, were clean streaked at 30°C for whole-genome sequencing onto M17-lac agar plates.
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