Draft Genome Sequences of Enterococcus faecalis Strains Isolated from Healthy Japanese Individuals.

Kazuki Tanaka,Masaru Tomita,Mitsuhiko P Sato,Kumiko Kato,Shinji Fukuda,Tomoya Tsukimi,Tsubasa Watabe,Toshitaka Odamaki,Julie C Dunning Hotopp

doi:10.1128/mra.00832-19

Abstract

Enterococcus faecalis is a common commensal of the intestines of humans and other mammals but is also a frequent cause of serious ailments. Here, we report 14 draft genome sequences of strains of Enterococcus faecalis, a normal inhabitant and Gram-positive bacterium that was isolated from 7 healthy Japanese volunteers.

Highlights

Enterococcus faecalis is a common commensal of the intestines of humans and other mammals
GenBank files of 25 E. faecalis strains were downloaded from the NCBI FTP site on 25 April 2019
The core-genome phylogeny indicated that the clade consisting of the strains isolated from the Japanese volunteers was divided into a clade close to the L12 strain and a clade close to the D32 strain (Fig. 1), which are pig-derived bacteria (GenBank accession numbers CP018102 and CP003726, respectively)

Summary

Introduction

Enterococcus faecalis is a common commensal of the intestines of humans and other mammals. GenBank files of 25 E. faecalis strains were downloaded from the NCBI FTP site on 25 April 2019. Nucleotide sequence alignments for core genes were produced using Roary v3.12.0 [8], with a minimum blastp percentage identity of 95, and MAFFT v7.407 [9].

Results

Conclusion