Abstract
We present the draft genome sequences of two clinical strains of Aspergillus turcosus, one azole-susceptible (strain HMR-AF-23/LSPQ-01275) and the other azole-resistant (strain HMR-AF-1038/LSPQ-01280), isolated from bronchoalveolar lavage fluid of two adult patients. These two strains are the first reported clinical isolates of A. turcosus.
Highlights
Invasive aspergillosis is a serious fungal infection of major concern in immunocompromised patients and is associated with a high mortality rate [1,2,3,4]
Mitochondrial sequences were discarded by mapping the cleaned reads onto the A. fumigatus mitochondrial genome with SMALT v0.7.6
For HMR-AF-1038, 9,174 genes were predicted, from which 830 have functional annotation. The completeness of these annotations was assessed by comparing the number of conserved orthologs to those of A. fumigatus, the closest reference species, using the tool BUSCO v2.0 [21] run with the Dikarya data set
Summary
Invasive aspergillosis is a serious fungal infection of major concern in immunocompromised patients and is associated with a high mortality rate [1,2,3,4]. Whole-genome sequencing was performed at the provincial public health laboratory. The Nextera XT library preparation kit (Illumina, Inc.) was used to prepare sequencing libraries for both strains. They were sequenced with a MiSeq 600-cycle sequencing kit v3 as paired-end reads (2 ϫ 300 bp) on a MiSeq sequencing platform (Illumina).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
